| 0.25% Trypsin + 0.02% EDTA ( 3 min; 37º C) | DMEM (4.5 g/L glucose) + 10% FBS + 2 mM L-Glutamine | Fibroblast- like | Kidney | African Green Monkey | African Green Monkey Kidney | Vero | IBRC C10001 |
| 0.25% Trypsin + 0.02% EDTA ( 3 min; 37º C) | DMEM (4.5 g/L glucose) + 10% FBS + 2 mM L-Glutamine | Fibroblast- like | Skin | Bovine | Bovine Skin Fibroblast | Bo01 | IBRC C10003 |
| 0.25% Trypsin + 0.02% EDTA ( 3 min; 37º C) | DMEM (4.5 g/L glucose) + 10% FBS + 2 mM L-Glutamine | Fibroblast- like | Skin | Sheep | Sheep Skin Fibroblast | Sh01 | IBRC C10030 |
| 0.25% Trypsin + 0.02% EDTA ( 3 min; 37º C) | DMEM (4.5 g/L glucose) + 10% FBS + 2 mM L-Glutamine | Fibroblast- like | Skin | Bovine | Bovine Skin Fibroblast | Bo02 | IBRC C10031 |
| 0.25% Trypsin + 0.02% EDTA ( 3 min; 37º C) | DMEM (4.5 g/L glucose) + 10% FBS + 2 mM L-Glutamine | Fibroblast- like | Skin | Goat | Goat Skin Fibroblast | Go01 | IBRC C10032 |
| Transplantation of cells from one culture vessel to another | RPMI1640 + 10% FBS + 2 mM L-Glutamine | Lymphoblast & Fibroblast - like | Blood | Cotton-top Tamarin Monkey | Monkey Lymphocyte | B95-8 | IBRC C10050 |
| Remove the cells mechanically Using cell scraper | DMEM (4.5 g/L glucose) + 10% FBS + 2 mM L-Glutamine | Macrophage | Peituneal cells | Mouse | Mouse Monocyte Macrophage | Raw 264.7 | IBRC C10072 |
| Detach cells mechanically by cell scraper after loosening by incubating in PBS (without Ca2+ or Mg2+) for 10-15 min | Iscove's MDM + 10% FBS + 2 mM L-Glutamine + 2.5 x 10[-5] M mercaptoethanol | Macrophage-like | Blood, peripheral; leukemia | Mouse | Mouse Myelomonocytic Leukemia | WEHI-3B | IBRC C10075 |
| Detach cells mechanically by cell scraper after loosening by incubating in PBS (without Ca2+ or Mg2+) for 10-15 min | DMEM (4.5 g/L glucose) + 10% FBS + 2 mM L-Glutamine + 2.5 x 10[-5] M mercaptoethanol | Macrophage-like | Blood | Mouse | Mouse Myelomonocytic Leukemia | WEHI-3B* | IBRC C10084 |
| 0.25% Trypsin + 0.02% EDTA ( 3 min; 37º C) | DMEM (4.5 g/L glucose) + 10% FBS + 2 mM L-Glutamine | Fibroblast-like | Embryo | Mouse | Mouse Embryo cell | NIH3T3 | IBRC C10100
|
| 0.25% Trypsin + 0.02% EDTA ( 3 min; 37º C) | RPMI 1640 + 10% FBS + 2 mM L-Glutamine | Epithelial- like | Pancreas | Rat | Rat Beta Cell Tumor | RIN-5F | IBRC C10101 |
| 0.25% Trypsin + 0.02% EDTA (3 min; 37º C) | MEM + 10% FBS + 2 mM L-Glutamine + 1mM Non-essential amino acids + 1mM Sodium Pyruvate | Fibroblast - like | Connective tissue; normal; subcutaneous; areolar and adipose | Mouse (strain C3H/An) | Mouse connective tissue fibroblast | L-929 | IBRC C10102 |
| Addition or replacement of fresh medium | DMEM (4.5 g/L glucose) + 10% FBS + 4 mM L-Glutamine | Lymphoblast | Spleen | Mouse | Mouse B Cell hybridoma | Sp2/0-Ag14 | IBRC C10106 |
| Remove the cells mechanically Using cell scraper | Grace + 10% FBS + 2 mM L-Glutamine + 2 mM Non-essential amino acids | Spherical | Ovary | Insect | Insect Ovary cell line | Sf9 | IBRC C10108 |
| 0.25% Trypsin + 0.02% EDTA (30seconds; 37º C) | DMEM (4.5 g/L glucose) + 15% Horse Serum + 2.5% FBS + 2 mM L-Glutamine | Cells growing as multilayers | Pancreas | Mouse | Mouse Pancreas Insulinoma (beta-tumor) | BETA-TC-3 | IBRC C10109 |
| Addition or replacement of fresh medium | DMEM (4.5 g/L glucose) + 20% Horse Serum + 2 mM L-Glutamine | Lymphoblast | Blood | Mouse | Mouse BALB/C Myeloma | MPC-11 | IBRC C10138 |
| 0.25% Trypsin + 0.02% EDTA (5 min or longer) | DMEM (4.5 g/L glucose) + 10% FBS + 2 mM L-Glutamine | Fibroblast-like | Kidney | African Green Monkey | African Green Monkey Kidney Cell Line, SV40 transformed | COS-7 | IBRC C10146 |
| 0.25% Trypsin + 0.02% EDTA (5 min or longer) | DMEM (4.5 g/L glucose) + 10% (FBS) + 2 mM L-Glutamine | Fibroblast-like | Embryo | Mouse | Mouse Embryo | 3T3L1 | IBRC C10152 |
| 0.25% Trypsin + 0.02% EDTA (3 min; 37º C) | DMEM:Hams F12 + 10% FBS + 2 mM L-Glutamine + 15 mM HEPES | Fibroblast- like | Breast; mammary gland | Mouse | Mouse Mammary Adenocarcinoma | MC4-L2 | IBRC C10167 |
| 0.25% Trypsin + 0.02% EDTA ( 3 min; 37º C) | DMEM (4.5 g/L glucose) + 10% FBS + 2 mM L-Glutamine + 1mM Non-essential amino acids | Epithelial-like | Embryo | Mouse | Mouse Embryonal Carcinoma | P19 | IBRC C10213 |
| 0.25% Trypsin + 0.02% EDTA ( 3 min; 37º C) | DMEM (4.5 g/L glucose) + 15% FBS + 2 mM L-Glutamine + 50 μM Mercaptoethanol + 20 mM HEPES | Epithelial - like | Pancreas | Mouse | Mouse Insulinoma | MIN6 | IBRC C10524 |
| 0.25% Trypsin + 0.02% EDTA (3 min; 37º C) | DMEM: F12 + 10% FBS + 2 mM L-Glutamine | Epithelial - like | Kidney | Dog | Canine Kidney Epithelial Cells | MDCK | IBRC C10556 |
| 0.25% Trypsin + 0.02% EDTA ( 3 min; 37º C) | DMEM (4.5 g/L glucose) + 10%FBS + 2 mM L-Glutamine | Fibroblast- like | Embryo | Mouse | Mous Embryo Neuroblast | B104-1-1 | IBRC C11310 |
| Remove the cells mechanically Using cell scraper | DMEM (4.5 g/L glucose) + 10% FBS + 2 mM L-Glutamine | Monocyte/Macrophage | Abelson murine leukemia virus-induced tumor; ascites | Mouse | Mouse Monocyte Macrophage | Raw 264.7 | IBRC C11324 |
| 0.25% Trypsin + 0.02% EDTA ( 3 min; 37º C) | DMEM (4.5 g/L glucose) + 10% FBS + 2 mM L-Glutamine | Fibroblast- like | Bone, calvaria | Mouse | Bone, Calvaria | MC3T3-E1 | IBRC C11328 |
| 0.25% Trypsin + 0.02% EDTA ( 3 min; 37º C) | DMEM (4.5 g/L glucose) + 10% FBS + 2 mM L-Glutamine | Fibroblast- like | Quail embryo | Quail | Quail Embryonal Fibroblast | Quail-01 | IBRC C11351 |
| 0.25% Trypsin + 0.02% EDTA ( 3 min; 37º C) | DMEM (4.5 g/L glucose) + 10% FBS + 2 mM L-Glutamine | Fibroblast- like | Quail embryo | Quail | Quail Embryonal Fibroblast | Quail-02 | IBRC C11352 |
| 0.25% Trypsin + 0.02% EDTA ( 3 min; 37º C) | DMEM (4.5 g/L glucose) + 10% FBS + 2 mM L-Glutamine | Fibroblast- like | Quail embryo | Quail | Quail Embryonal Fibroblast | Quail-03 | IBRC C11353 |
| 0.25% Trypsin + 0.02% EDTA ( 3 min; 37º C) | DMEM (4.5 g/L glucose) + 10% FBS + 2 mM L-Glutamine | Fibroblast- like | Quail embryo | Quail | Quail Embryonal Fibroblast | Quail-04 | IBRC C11354 |
| 0.25% Trypsin + 0.02% EDTA ( 3 min; 37º C) | DMEM (4.5 g/L glucose) + 10% FBS + 2 mM L-Glutamine | Fibroblast- like | Quail embryo | Quail | Quail Embryonal Fibroblast | Quail-05 | IBRC C11355 |
| 0.25% Trypsin + 0.02% EDTA ( 3 min; 37º C) | DMEM (4.5 g/L glucose) + 10% FBS + 2 mM L-Glutamine | Fibroblast- like | Quail embryo | Quail | Quail Embryonal Fibroblast | Quail-06 | IBRC C11356 |
| 0.25% Trypsin + 0.02% EDTA ( 3 min; 37º C) | DMEM (4.5 g/L glucose) + 10% FBS + 2 mM L-Glutamine | Fibroblast- like | Quail embryo | Quail | Quail Embryonal Fibroblast | Quail-07 | IBRC C11357 |
| 0.25% Trypsin + 0.02% EDTA (3 min or longer) | DMEM (4.5 g/L glucose) + 10% FBS + 2 mM L-Glutamine | Epithelial-like | Distal tubule kidney | Dog | Madin–Darby canine kidney transfected with the MDR1 gene | MDCK-MDR1 | IBRC C11434 |
| Remove the cells mechanically Using cell scraper | DMEM (4.5 g/L glucose) + 10% FBS + 2 mM L-Glutamine | Macrophage | Peituneal cells | Mouse | Mouse Monocyte Macrophage | Raw 264.7-GFP+ | IBRC C11438 |
| 0.25% Trypsin + 0.02% EDTA ( 3 min; 37º C) | DMEM (4.5 g/L glucose) + 10% FBS + 2 mM L-Glutamine | Epithelial- like | Ovary | Chinese Hamster | Chinese Hamster Ovary Epithelium | CHO | IBRC C10028 |
| 0.25% Trypsin + 0.02% EDTA (2 min; 37º C) | Hams F12 + 10% FBS + 2 mM L-Glutamine | Fibroblast - like | Ovary | Chinese Hamster | Chinese Hamster Ovary Fibroblastoid Cell | CHO-K1 | IBRC C10136 |
| 0.25% Trypsin + 0.02% EDTA ( 3 min; 37º C) | DMEM (4.5 g/L glucose) + 10% FBS + 2 mM L-Glutamine | Fibroblast- like | Skin | Rabbit | Rabbit Skin Fibroblast | Ra 01 | IBRC C10069 |
| 0.25% Trypsin + 0.02% EDTA ( 3 min; 37º C) | DMEM (4.5 g/L glucose) + 10% FBS + 2 mM L-Glutamine | Fibroblast- like | Skin of tail | Rat | Rat Skin Fibroblast | RWE01 | IBRC C10074 |
| 0.25% Trypsin + 0.02% EDTA ( 3 min; 37º C) | DMEM (4.5 g/L glucose) + 10% FBS + 2 mM L-Glutamine | Fibroblast- like | Skin of ear | Rat | Rat Skin Fibroblast | RWT01 | IBRC C10076 |
| 0.25% Trypsin + 0.02% EDTA ( 3 min; 37º C) | DMEM (4.5 g/L glucose) + 10% FBS + 2 mM L-Glutamine | Fibroblast- like | Skin of ear | Rat | Rat Skin Fibroblast | RWT02 | IBRC C10077 |
| 0.25% Trypsin + 0.02% EDTA ( 3 min; 37º C) | DMEM (4.5 g/L glucose) + 10% FBS + 2 mM L-Glutamine | Fibroblast- like | Skin of tail | Rat | Rat Skin Fibroblast | RWE02 | IBRC C10078 |
| 0.25% Trypsin + 0.02% EDTA (5 min or longer) | DMEM:F12 + 15% FBS + 2 mM L-Glutamine | Fibroblast- like | Renal | Rat | Rat Renal Mesangial cells | RRC-01 | IBRC C11212 |
| 0.25% Trypsin + 0.02% EDTA ( 3 min; 37º C) | DMEM (4.5 g/L glucose) + 10% FBS + 2 mM L-Glutamine | Fibroblast- like | Skin | Rat | Rat Skin | RWD01 | IBRC C11231 |
| 0.25% Trypsin + 0.02% EDTA (5 min or longer) | DMEM:F12 + 10%FBS + 2 mM L-Glutamine | Fibroblast- like | Renal | Rat | Rat Renal Mesangial Cells | RRC-02 | IBRC C11232 |
| 0.25% Trypsin + 0.02% EDTA ( 3 min; 37º C) | DMEM (4.5 g/L glucose) + 10% FBS + 2 mM L-Glutamine | Fibroblast- like | Skin | Rat | Rat Tail | RTTF-01 | IBRC C11233 |
| 0.25% Trypsin + 0.02% EDTA ( 3 min; 37º C) | DMEM (4.5 g/L glucose) + 10% FBS + 2 mM L-Glutamine | Fibroblast- like | Tail Skin | Mouse | Mouse Skin Fibroblast | MTTF-01 | IBRC C11234 |
| 0.25% Trypsin + 0.02% EDTA ( 3 min; 37º C) | DMEM (4.5 g/L glucose) + 10% FBS + 2 mM L-Glutamine | Fibroblast- like | Back Skin | Mouse | Mouse Skin Fibroblast | Mo04 | IBRC C11235 |
| 0.25% Trypsin + 0.02% EDTA ( 3 min; 37º C) | DMEM (4.5 g/L glucose) + 10%FBS + 2 mM L-Glutamine | Fibroblast- like | Embryo | Mouse | Mouse Embryo Neuroblast | B104-1-1 | IBRC C11310 |
| 0.25% Trypsin + 0.02% EDTA ( 3 min; 37º C) | DMEM (4.5 g/L glucose) + 10% FBS + 2 mM L-Glutamine | Fibroblast- like | Skin | Mouse | Mouse Skin Fibroblast | mFib-1 | IBRC C11345 |
| 0.25% Trypsin + 0.02% EDTA ( 3 min; 37º C) | DMEM (4.5 g/L glucose) + 10% FBS + 2 mM L-Glutamine | Fibroblast- like | Skin | Mouse | Mouse Skin Fibroblast | mFib-2 | IBRC C11346 |
| 0.25% Trypsin + 0.02% EDTA ( 3 min; 37º C) | DMEM (4.5 g/L glucose) + 10% FBS + 2 mM L-Glutamine | Fibroblast- like | Skin | Mouse | Mouse Skin Fibroblast | mFib-4 | IBRC C11348 |
| 0.25% Trypsin + 0.02% EDTA ( 3 min; 37º C) | DMEM (4.5 g/L glucose) + 10% FBS + 2 mM L-Glutamine | Fibroblast- like | Skin | Mouse | Mouse Skin Fibroblast | mFib-3 | IBRC C11349 |
| 0.25% Trypsin + 0.02% EDTA (3 min or longer) | DMEM (4.5 g/L glucose) + 10% FBS + 2 mM L-Glutamine | Fibroblast-like and flat | Eye (retinal pigmented epithelium; retina) | Rat | Rat Normal Retina Eyes | Muller | IBRC C11430 |
| 0.25% Trypsin + 0.02% EDTA ( 3 min; 37º C) | DMEM:F12 + 10% FBS + 2 mM L-Glutamine + 1mM Non-essential amino acids + 1mM Sodium Pyruvate | Fibroblast- like | Skin | Cat | Cat Skin Fibroblast | CFC-01 | IBRC C11481 |
| 0.25% Trypsin + 0.02% EDTA ( 3 min; 37º C) | DMEM:F12 + 10% FBS + 2 mM L-Glutamine | Fibroblast- like | Skin | Rabbit | Rabbit Skin Fibroblast | Ra 02 | IBRC C11486 |
| 0.25% Trypsin + 0.02% EDTA ( 3 min; 37º C) | DMEM:F12 + 15% FBS + 10% Horse Serum + 2 mM L-Glutamine | Fibroblast- like | Thigh muscle | Rabbit | Rabbit Muscle Fibroblast | RaMu-01 | IBRC C11487 |
